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For a 10mg peptide vial, add 2mL for a 5mg/mL concentration (500mcg per 0.1mL), 5mL for a 2mg/mL concentration (200mcg per 0.1mL, most commonly recommended), or 10mL for a 1mg/mL concentration (100mcg per 0.1mL, optimized for long-term stability)

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If left untreated, vitamin B12 deficiency can lead to anemia, nerve damage and more, warns Taylor

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The main drawback of DNP-based SSNMR techniques for protein studies is related to the use of cryogenic temperatures, causing an inhomogeneous line broadening due to the presence of numerous protein conformations at low temperature