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Pharmacological mechanism is well-described in the peer-reviewed literature

233 Procyanidins Procyanidins, polyphenolic compounds abundant in grape seeds, blueberries, and other fruits, 234 are recognized for their antioxidant properties

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PDE5 inhibitors are contraindicated in patients taking nitrates for angina and the medication riociguat

In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins