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All animals were housed under a 12-h light12-h dark cycle at an ambient temperature of 22 2 C and 4565% relative humidity, with ad libitum access to standard rodent chow and water

They may spend time either in the closed area or in the open area

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Some protocols split the daily dose into morning and evening injections for more consistent tissue levels, taking advantage of the peptide's short half-life by maintaining more frequent, lower doses