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Gene expression analysis using the Broad Institute Connectivity Map The authors analysed how GHK-Cu affects human gene expression using the Connectivity Map (CMap) a massive database developed at MIT and Harvard that maps how compounds alter gene activity

This phenomenon is thought to be due to the inability of cells to restore normal GSH levels or to increased production of reactive oxygen species (ROS), which can damage macromolecules fundamental to cell survival before being deactivated by antioxidant defenses

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Then we used IHC to detect the expression and distribution of DDIT4 in mouse liver tissues

26 Mechanisms of Action Free Radical Scavenging: Antioxidants present in medicinal plants have the ability to counter excess ROS produced in excess under hyperglycaemic conditions