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Effect of immobilized cell-binding peptides on chitosan membranes for osteoblastic differentiation of mesenchymal stem cells

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HUBERMAN: In order to find out the lethal dose of something, as you can imagine, unfortunately, the way these studies are done is they give animals more and more, that is higher and higher doses of a given compound, find out at what point about 50% of the population of those animals starts to die, and then that's the so-called LD50, or at least that's one crude way of describing it

One critical caveat: animal LD50 studies do not guarantee human safety

The procedure involves injecting a small amount of anesthetic or saline into the trigger point to relax the muscle