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Key factors of GSH synthesis are the activity of the rate-limiting enzyme, glutamateCys ligase, and the availability of Cys

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The last GSH synthetic process is the one depending on the conversion of the oxidized dimer GSSG to 2 reduced GSH molecules in cells by glutathione reductase (GR), an ubiquitous enzyme of the family of disulfide reductases, in the presence of NADPH and flavin adenine dinucleotide -FAD-

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